Senior Research Assistant II/ Lab Manager | Boston, MA

Detailed Information

  • Location: Boston, MA

  • Company: Joslin Diabetes Center

and liver biology and tissue secreted exosomal mi RNAs using in vivo and in vitro models, including differentiated human i PS cells. The Senior Research Assistant II/Lab Manager will also function as a leader and resource in the Kahn lab. Thus, additional responsibilities will include: overseeing laboratory organization, equipment and supplies; working independently, as well as cooperatively, with research fellows and students; performance of biochemical, enzymatic, molecular, immunological and physiological tests, including western blotting and q PCR; some experiments may involve handling small lab animals and collection and processing tissue and occasional use of radioactive tracers; maintaining

and organizing records; calculating, compiling and graphing data; and preparation of presentations and reports.

Responsibilities: General laboratory procedures and laboratory management Must be adept at tissue culture, including cells requiring differentiation in culture, such as i PS cells or preadipocytes Cell-based assays Performance of q PCR, western blotting, ELISAs, and biochemical assays Experience in DNA manipulation a benefit Some studies may involve mice, including weighing and taking tail blood, physiological testing, and tissue collection, as well as genotyping and maintenance of transgenic animal colonies Organization and presentation of data for reports and in laboratory

meetings, including registration of lab protocols with Harvard COMS (Committee on Microbiological Safety) Work with the JDC Safety Office to ensure compliance with all Safety protocols Maintain a clearly written and organized experimental electronic notebook, and assist in the generation and maintenance of various databases Oversee maintenance of laboratory equipment and order supplies as necessary Work collaboratively with others in the lab, as well as members of other research groups at the Joslin and with our collaborators at other institutions Effectively organize work schedule to accomplish the completion of multiple tasks or multiple experimental procedures Take responsibility for planning experiments and contributing to the overall organization of the laboratory in order to effectively utilize work time Dispose of chemical and biohazardous wastes safely Maintain records of radioactivity use and disposal Other duties as assigned Qualifications: Bachelor of Science with extensive experience, Master's Degree in Biological Sciences, Biochemistry, or higher preferred Minimum 5 years of demonstrated practical experience with relevant laboratory methods Experience working with mice Experience with cell culture and in vitro differentiation techniques Knowledge of standard protein chemistry and molecular biology techniques, and some experience with DNA engineering Advanced knowledge of software for analysis of scientific data, as well as Microsoft Office Suite (Excel, Power Point) and Graph Pad Detail oriented, with the ability to prioritize responsibilities - through completion Ability to work both independently and collaboratively with other members of the lab, professional staff, and external contacts Strong verbal and written communication skills Strong organizational skills Joslin is an Equal Opportunity Employer of Minority/Female/Individuals with Disabilities/Protected Veterans.

Joslin values diversity and inclusion, and we encourage diversity applicants. Joslin Diabetes Center is an Equal Opportunity Employer and it is the policy of Joslin to prohibit discrimination of any type and to afford equal employment opportunities to employees, and all qualified applicants will receive consideration for employment without regard to race, color, religion, interaction, interactionual orientation, gender identity, national origin, age, genetic information, marital/parental status, disability status, protected veteran status or any other status protected by law. PDN-9acdb117-eebe-41ba-81a9-25da5123b655

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