Location: San Francisco, CA
Company: Pasturebio
emissions from fermentation in ruminants and promoting effective digestion. This position provides a unique opportunity to contribute to cutting-edge research and deepen our understanding of antigen design, immune responses, and vaccinology in the veterinary field.
Responsibilities Design and engineer novel antigens based on an understanding of immunological principles and vaccine targets. Synthesize m RNA using in vitro transcription methods to generate RNA transcripts for various applications such as in vitro translation, transfection, and functional studies. Primer designing, cloning, expressing, and purifying recombinant proteins and antigens using molecular biology techniques. Develop
and optimize expression systems for high-yield production of antigens. Conduct molecular characterization and validation of expressed antigens. Develop and perform immunological assays to evaluate antigen-antibody interactions, such as ELISA, SPR, and FACS.
Culture and isolate microorganisms ( e. g. bacteria and archaea) to identify them and run tests to determine how they function. Prepare and dispense solutions and chemicals for lab needs, including reagents, acids, bases, buffers, and stains. Design and execute immunization schedules in animal experimental models to evaluate antigen immunogenicity, vaccine efficacy, and in vivo immune responses. Hands-on tissue homogenization, dissociation,
and preparation techniques for DNA, RNA, protein extraction, and cell harvesting.
DNA (Sanger) and RNA sequencing including an understanding of sample preparation and sequencing platforms for accurate mutation identification and gene expression analysis. Visualizing specific proteins within tissues with techniques like immunohistochemistry (IHC) and immunofluorescence (IF). Collaborate with cross-functional teams to integrate antigen design and characterization into broader research and development projects. Work closely with bioinformaticians, immunologists, and other scientists to analyze data and derive meaningful insights. Maintain detailed records of experiments, protocols, and results.
Prepare and present scientific findings internally and, when necessary, externally at conferences, scientific meetings, peer-reviewing manuscripts, and patent applications. Data Interpretation, analyze complex data sets, generate reports, and present findings to internal stakeholders and external partners. Contribute with management and collaborations with external resources CRO and research partners. Qualifications Ph. D. in Molecular Biology, Veterinary Research, Immunology, Biochemistry, or related field, with a focus on antigen design and protein sciences with 2-3 years experience.
Expertise and hands-on experience in antigen design, precision RNA synthesis, innovative transfection techniques, and quality control Proficiency in molecular biology techniques including hands-on protein expression, m RNA development, DS-PAGE, analytical SEC, western blot, q PCR, RT-PCR and purification methods. Hands-on experience in immunological assays and protein characterization techniques, such as ELISA, ELISpot SPR, Luminex, MSD, and multicolor flow cytometry. Cultivate and propagate viable pathogenic and nonpathogenic stock cultures of bacteria, viruses, and fungi.
Excellent data analysis, presentation, problem-solving skills, and written and oral communication skills Ability to work collaboratively in a team-oriented environment and cross-functionally Experience using Excel and Graphpad Prism and Knowledge of statistics methods. Good to Have Familiarity with methane-producing microorganisms and their metabolic pathways, and methods to quantify methane emission by ruminants in vivo and in vitro. We offer competitive compensation, excellent benefits, and opportunities for career advancement. Join us in our mission to make significant contributions to the field of vaccine development to mitigate methane emissions in ruminants.
The candidate should be authorized to work in the United States without visa sponsorship. Equal Employment Opportunity Pasture Biosciences is committed to providing equal opportunity and a work environment free of discrimination. All qualified applicants will receive consideration for employment without regard to race, color, religion, marital status, national origin, ancestry, interaction, interactionual orientation, gender identity, disability, medical condition, pregnancy, protected veteran status, age, citizenship, or any other characteristic protected by applicable laws. #J-18808-Ljbffr
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and post-analytic procedures on biological specimens to ais health care providers in the diagnosis, treatment, monitoring and prevention of disease. Job Description: JOB ACCOUNTABILITIES: PRE-ANALYTIC, ANALYTIC AND POST-ANALYTIC WORK: Under direct supervision of a Clinical Lab Scientist (CLS), performs procedures to complete test order entry, specimen collection and specimen processing/handling.
--- Performs analyses of biological specimens using automated and manual test methods of waived or moderate complexity (per CLIA definition), producing test results necessary for the diagnosis, treatment, monitoring and prevention of disease. --- Performs post-analytic procedures, including interpretation,
releasing, reporting results; amending/correcting results, responding to inquiries, and specimen storage and retrieval. Recognizes when patient results require additional action prior to reporting (e.
g. delta checks and critical values), taking action appropriately. EQUIPMENT: Calibrates and adjusts laboratory equipment according to requirements and manufacturer's guidelines, including providing regular preventive maintenance (where automated methods are used) of analytical equipment and performing minor adjustments and repairs. QUALITY ASSURANCE: Scope of Quality Assurance (QA) duties and tasks includes maintaining a safe and orderly work area, inventory management, quality control,
proficiency testing, record keeping, privacy, security and confidentiality, and continual process improvement.
Recognizes when test systems are not functioning within control, taking action appropriately. JOB KNOWLEDGE & CRITICAL THINKING: Maintains up-to-date operational information and job knowledge; effectively applies information, job knowledge and critical thinking to make sound decisions and solve problems within scope of job responsibilities; escalates problems appropriately if outside of job scope. Completes competency backssment activities as assigned. CUSTOMER SUPPORT: Contributes to the satisfaction of internal and external customers by responding to and resolving - inquiries, complaints, and problems; or by escalating appropriately if problem is outside of job scope.
TRANSFUSION SERVICE: If assigned to the Transfusion Service, performs various duties under direct supervision of a CLS. --- Manages blood product inventory, maintaining adequate working and safety stock. --- Performs pre-analytic, analytic and post-analytic procedures for moderate complexity testing. --- Prepares and issues blood products for transfusion. COMPLIANCE: Adheres to applicable laws, regulations, codes, accreditation standards, policies, processes and procedures EDUCATION: -Associate's Degree CERTIFICATION & LICENSURE: MLT-Medical Laboratory Technician Upon Hire SKILLS AND KNOWLEDGE: Demonstrated knowledge and technical competence in routine specimen collection, preparation, testing applications, laboratory operations, and quality assurance in one or more assigned clinical specialty areas; examples: Chemistry, Special Chemistry, Hematology (no microscopy), Urinalysis (no microscopy) and Coagulation.
Quantity of Work: Ability to organize and prioritize job duties and assigned tasks for completion within expected time-frames Proficiency: Quality of Work: Ability to accurately, precisely, and reliably perform job duties and assigned tasks.
Demonstrates sound judgment and problem solving relevant to assigned duties. Proficiency: Culturally Competent Communication: Ability to compassionately and effectively interact with patients of diverse ages, backgrounds, values, beliefs and behaviors Proficiency: Workflow Management Ability to appropriately respond to: 1) fluctuations in volume; 2) unexpected situations or problems such as equipment or information systems failure; 3) shift-to-shift hand-offs; 4) STAT test orders; 5) specimen integrity issues 6) regular review of pending logs, priority assignments, etc.
Proficiency: Adherence to Practices and Procedures: Ability to follow standard practices, processes, and procedures in sequence. Knowledge of applicable local/state/federal regulations, codes, policies, and procedures aimed at ensuring the privacy and safety of patients. Ability to use computers, including Microsoft Office suite, electronic mail, internet, etc. -Job Shift: Days Schedule: Per Diem/Casual Shift Hours:8Days of the Week: Variable Weekend Requirements: As Needed Benefits: No Unions: No Position Status: Non-Exempt Weekly Hours:0Employee Status: Per Diem/Casual Number of Openings:1Sutter Health is an equal opportunity employer EOE/M/F/Disability/Veterans.
Pay Range is $50.35 to $62.93 / hour The salary range for this role may vary above or below the posted range as determined by location. This range has not been adjusted for any specific geographic differential applicable by area where the position may be filled. Compensation takes into account several factors including but not limited to a candidate's experience, education, skills, licensure and certifications, department equity, training and organizational needs.
Base pay is just one piece of the total rewards program offered by Sutter Health. Eligible roles also qualify for a comprehensive benefits package. About Us Working at Sutter Health #J-18808-Ljbffr
